KIF26B knockdown inhibits cancer cell proliferation. Western blot analysis of KIF26B protein expression in stable knockdown RKO and HT29 cell lines. Grayscale values were evaluated (n = 3, *P <0.05) (A). Expression of proliferation-related genes was inhibited in KIF26B knockdown cells according to real-time PCR analysis (n = 3; *P < 0.05) (B). Effects of KIF26B knockdown on cell growth was evaluated by Cell Counting Kit-8 assays (C) and plate colony formation assays (D) (n = 3; *P < 0.05).