DCA promotes oxidative phosphorylation through inhibiting glycolysis and attenuates PENAO-induced acid production. Two metabolic parameters, OCR and ECAR, were measured in (A) U87 cells and (B) BAH1 cells after 24 hr treatment with 5 μM PENAO, 20 mM DCA and the combination. Results are representative of three independent experiments and presented as means ± s.d. of 3–5 measurements. *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.001 vs PENAO.