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Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: Oncolytic tanapoxvirus expressing FliC causes regression of human colorectal cancer xenografts in nude mice

Figure 2

Verification of ablation in recombinant TPVs. Agarose gel electrophoresis (1%) were done to verify the ablation of the viral 2L and 66R genes. Each viral DNA was probed for sequences internal to the knocked-out 2L and 66R region. An ablated gene will show no band. The 2L region predicted amplicon is 904 bp; the 66R region predicted region is 379 bp; a region within the 136R gene was used as a control and had a predicted amplicon length of 531 bp. Lane a: molecular weight marker 2-log ladder (New England Biological); Lane b: TPV/egfp; Lane c: TPV/Δ66R/mGM-CSF (66R is ablated); Lane d: TPV/Δ66R/mCCL2 (66R is ablated); Lane e: TPV/Δ66R/fliC (66R is ablated); Lane f: TPV/Δ66R/fliC (66R is ablated); Lane g: TPV/Δ2L (2L is ablated); Lane h: TPV/Δ2L/Δ66R/fliC (both 2L and 66R are ablated).

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