Figure 4

FliC expression in recombinant TPV /Δ 66R/ fliC TPV /Δ 2L /Δ 66R/ fliC . The viruses TPV/Δ66R/fliC TPV/Δ66R/Δ2L/fliC were inoculated into 60 mm dishes onto semi-confluent OMK cells at 10 pfu/cell and cytoplasmic extracts at 3, 5 and 6 days post-infection were subjected to 12% SDS-PAGE and Western blot analysis using a monoclonal antibody against FliC and a secondary anti-mouse HRP conjugated antibody. Visualization of bands was by ECL. Gel (a) lanes (a) mock infected, day 0; (b) mock infected, day 3; (c) wild-type TPV; (d) TPV/Δ66R, the “empty vector” control; (e) TPV/Δ66R/fliC; (f) TPV/egfp; (g) TPV/Δ66R/mGM-CSF; (h) TPV/Δ2L; (i) TPV/2L/Δ66R/fliC. Gel (b) lanes (a) FliC (53 kDa) purified from S. enterica, the positive control; (b) mock infected, day 6; (c) TPV/Δ66R/Δ2L/fliC, day 3 post-infection; (d) TPV/Δ66R/Δ2L/fliC, day 5 post-infection; (e) TPV/Δ66R/Δ2L/fliC, day 6 post-infection.