Figure 5

Virotherapy. Tumors were induced by subcutaneous injection of 5 × 10⁶ HCT 116 cells onto the dorsal surface of athymic nude mice. In each group n = 5 or 6 mice. Mice were randomly segregated into the control or experimental groups when tumor size reached 75 mm³. In each graph the y-axis is tumor volume (mm³) and the x-axis is time (days post virotherapeutic injection). (a ) A single mock injection containing 100 μl of vehicle only or recombinant virus was administered at day 0 and tumor volume was measured at three-day intervals. Tumor volume was calculated using the formula (length) × (width) × (height) × (π/6). Average tumor volume is shown (black filled squares for each experimental group). Bars show the standard error of the mean (±1 SEM). Points indicated with an asterisk (*) are significantly reduced from the control (p ≤ 0.05). (a) Mock virotherapeutic injection (vehicle only, open gray circles). This is the group to which all experimental groups were compared to test for OV virotherapeutic effect upon average tumor volume. (b) TPV/egfp; (c) TPV/Δ66R/mMCP-1; (d) TPV/Δ66R/mGM-CSF; (e) TPV/Δ66R/fliC; (f) TPV/Δ66R; (g) TPV/Δ2L; (h) TPV/Δ2L/Δ66R/fliC.