Journal of Experimental & Clinical Cancer Research

Table 2 Viruses made using the p2KO ablation/insertion vector

From: Oncolytic tanapoxvirus expressing FliC causes regression of human colorectal cancer xenografts in nude mice

TPV recombinant	Gene ablated	Gene added	Reporter (s)
TPV/egfp	---	EGFP
TPV/Δ66R	66R	---	mCherry
TPV/Δ66R/mGM-CSF	66R	mGM-CSF	mCherry
TPV/Δ66R/mMCP-1	66R	mMCP-1	mCherry
TPV/Δ66R/fliC	66R	fliC	mCherry
TPV/Δ2L	2L	---	mCherry
TPV/2L/Δ66R/fliC	66R, 2L	fliC	EGFP, mCherry

All viruses expressed the fluorescent reporter mCherry with the exception of TPV/egfp, which was modified to express fluorescent reporter EGFP but had no other genetic modifications. “Gene added” refers to an ORF added and expressed other than the fluorescent reporter. The TPV/Δ2L/Δ66R/fliC double-ablated recombinant TPV expressed both EGFP and mCherry.

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ISSN: 1756-9966

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