Figure 3

Icaritin induces ENKL cell apoptosis. Cell apoptosis of SNK-10 and SNT-8 was analyzed by flow cytometry and florescence microscopy, respectively, after 48 h treatment with Icaritin. A. Representative histograms of annexin V-FITC/PI double-staining flow cytometry. B. Percent apoptosis (including early and late apoptotic cells) determined by flow cytometry (n = 3). C. Cell apoptosis assessed by florescence microscopy. Cells were stained with Hoechst 33258. (a) Cell and nuclear morphology by florescence microscopy (magnification 400×). The arrows indicate the cells undergoing apoptosis; (b) Percent apoptosis determined by morphology-based cell sorting (n = 3). At least 1,000 cells from five randomly selected fields were counted for each data point. *p < 0.05, ^#p < 0.01 vs control.