Figure 3

HCS treatment upregulated the expression of Fas, Fasl and TNF-α. The mRNA levels of Fas, Fasl, TNF-α and TNFR1 in T24 (A) and EJ (B) cells after HCS treatment. (C) Quantification of TNF-α in conditioned cell supernatant using ELISA. (D) The protein levels of Fas, Fasl and TNFR1 in T24 and EJ cells after HCS treatment were detected by western bolt. *, P < 0.05 vs control group.