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Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: DEC2 expression is positively correlated with HIF-1 activation and the invasiveness of human osteosarcomas

Figure 2

The effects of DEC2 knockdown on HIF-1α protein levels in osteosarcoma cell lines. DEC2 was knocked down in U2OS, MNNG and 143B cell lines: Cells were transfected with indicated siRNAs and cultured for 48 h under either normoxia or hypoxia prior to cell harvest and protein sample collection. HIF-1α and DEC2 levels were determined by Western blotting, and β-actin was determined for normalization of sample loading.

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