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Figure 4 | Journal of Experimental & Clinical Cancer Research

Figure 4

From: Long noncoding RNA CCAT1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge

Figure 4

CCAT1 acts as a molecular sponge for let-7. (A) Bioinformatics predicted let-7 binding sites at two distinct positions in CCAT1. Partial sequences of CCAT1 and sequences of four let-7 subtypes are shown. Numbers are in nucleotides relative to the transcriptional start site of CCAT1. (B) Schematic outlining the MS2-RIP strategy to validate endogenous miRNA:CCAT1 binding. (C) MS2-RIP followed by miRNA qRT-PCR to detect miRNAs endogenously associated with CCAT1. (D) Expression levels of let-7 after the transfection of pcDNA3.1-CCAT1 or pcDNA3.1 into SMMC-7721 cells. (E) Luciferase activity in SMMC-7721 cells cotransfected with let-7 and luciferase reporters containing nothing, CCAT1 or mutant CCAT1. Data are presented as the relative ratio of firefly luciferase activity to renilla luciferase activity. (F) CCAT1 expression levels after the transfection of let-7 mimics or the miRNA negative control into SMMC-7721 cells. All values are presented as mean ± standard error based on at least three independent experiments. *p < 0.05, **p < 0.01.

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