Figure 5
Regulation of endogenous let-7 targets by CCAT1. (A) The expression of CCAT1 in stable SMMC-7721 cell clones. (B) HMGA2 and c-Myc mRNA levels in stable SMMC-7721 cell clones. (C) HMGA2 and c-Myc protein levels in stable SMMC-7721 cell clones. (D, E) The expression of CCAT1(D) or let-7b (E) in indicated HepG2 cell clones after transfection of let-7 inhibitor. (F) HMGA2 and c-Myc mRNA levels in stable HepG2 cell clones. (G) HMGA2 and c-Myc mRNA levels in stable HepG2 cell clones. For A-G, all values are presented as mean ± standard error based on at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. (H) The correlation between CCAT1 transcript level and c-Myc mRNA level was measured in 66 HCC tissues. The delta-Ct values (normalized to 18S rRNA) were subjected to Pearson correlation analysis. (I) Immunohistochemical scores of CCAT1 in HCC tissues of different CCAT1 expression (Mann–Whitney test, p < 0.001).