Figure 3
Overexpression of IGFBP-7 in A253-5 cell reverses EMT and suppress cell invasion. (A) Western blot results show up-regulated E-cadherin and down-regulated Vimentin after overexpression IGFBP-7 in A253-5 and reversed by siRNA knockdown. (B) Invasion assay after overexpression of IGFBP-7 in A253-5 cell. Images show that the cells invaded through the Matrigel coated membrane at a magnification of × 100. C: A253-5, Mock: A253-5 transfected with pEGFP-N1, IGFBP7: Transient IGFBP-7 expression A253-5 cells, IGFBP7 + NCsi: Transient IGFBP-7 expression A253-5 cells transfected with negative control siRNA, IGFBP7 + siRNA: Transient IGFBP-7 expression A253-5 cells transfected with siRNA against IGFBP7. * indicated p < 0.05, ** indicated p < 0.01, *** indicated p < 0.001 compared with A253-5 Mock cells. # indicated p < 0.01, ### indicated p < 0.001 compared with IGFBP7 + NCsi.