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Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: ARE/SUZ12 dual specifically-regulated adenoviral TK/GCV system for CML blast crisis cells

Figure 2

Basal and inducible SUZ12 activity in Bcr/abl+ cells. (A) Promoter activity assay of different SUZ12 upstream fragments via dual-luciferase reporter assay in Bcr/abl+PHA cells. Bcr/abl+PHA cells were transfected as depicted in Methods and luciferase activity was measured 48 h later. Data are presented as a fold-changes ± SD of firefly/Renilla (F/R) ratios, means ± SEM, n=3. (B) Promoter activity assay of SUZ12 or ARE/SUZ12 by ONE-Glo™ luciferase assay in K562, K562/G01, KCL22, and HepG2. Cells were transduced as depicted in Methods and luciferase activity was measured 72 h later. Data are normalized to protein and are presented as fold-increases versus Ad-control, means ± SEM, n=3. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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