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Figure 1 | Journal of Experimental & Clinical Cancer Research

Figure 1

From: SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells

Figure 1

SULF2 expression in prostate cancer cells. Normal prostate epithelial cell line RWPE-1, and prostate cancer cell lines LNCap, PC3 and DU-145 SULF2 mRNA level was analyzed by RT-PCR in agarose gel (A). RWPE-1, PC3 and DU-145 cells were transfected with either SULF2 expressing plasmid pcDNA3.1/Myc-His(−)Hsulf-2 (Addgene plasmid 13004) or empty vector using Fugene reagent (Promega). The control cells (CTRL) were not transfected. SULF2 mRNA expression was confirmed with quantitative real-time PCR. The expression level of each gene was normalized by GAPDH expression (B). The overexpression of SULF2 was also confirmed by protein western blotting under non-reducing conditions (C) and immunofluorescence analyzed in confocal microscope (D). The data from each experiment was obtained in triplicate and are represented as the average ± standard deviation. (CTRL: not transfected cells; VECTOR: cells transfected with the empty vector; SULF2: cells transfected with SULF2 containing vector). Scale bars 20 μm. *P ≤ 0.05.

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