Figure 4

Knockdown of SULF2 decreased viability and migration of prostate cells. RWPE-1 epithelial prostate cells and DU-145 and PC3 prostate cancer cells were transfected with siRNA (Life Technologies) targeting SULF2. To silence SULF2 gene, trials with siRNA preset by the manufacturer (Life Technologies, CA, USA) were performed as described in Methods. Three shRNAs were used to each gene, in addition to the positive (GAPDH) and negative (scramble sequence) controls. The gene silencing was confirmed by Real Time PCR 48 h after transfection (A). MTT viability assay (B) and wound healing assay (C) were performed as described previously. (CTRL NEG: scramble siRNA sequence). *P ≤ 0.05.