Figure 8

Cocultures of prostate cancer cells overexpressing SULF2 and stromal cells. Prostate cancer cells (PC3 or DU-145) were seeded inside the O-ring and fibroblasts stromal cells were seeded around the O-ring. The O-ring was removed and the cultures maintained in the same conditions until the cells filled the O-ring area. The cells were firstly visualized in phase contrast in optical microscope in bright field. Immunolocalization of actin, SULF2, vimentin, and fibronectin were performed after fixation of the cells with 2% formaldehyde. The nuclei (blue) were stained with DAPI. F: fibroblasts, P: prostate cancer cells. Scale bar represents 100 μm.