The expression of miR-184 in U87 and U251 cells after transfection modulated the expression of TNFAIP2. A. To keep a high transfection efficiency, qRT-PCR was used to detect the expression of miR-184 in U87 and U251 cells 48 h after transfection with miR-184 mimic, inhibitors, and the negative control miRNA. U6 was used as the loading control. B. shows the bioinformatic analysis using Targetscan, miRwalk, and miRanda, which predicted that miR-184 targeted TNFAIP2. C. shows the left panel, in which the Western blot analysis showed that miR-184 inhibited the expression of TNFAIP2 compared with the Mock group or the negative control miRNA group. The right panel shows the relative expression of TNFAIP2 protein in glioma cells, as depicted in the histogram. The protein expression levels were normalized to GAPDH. D. depicts the results of the qRT-PCR analysis, which showed that miR-184 inhibited the mRNA expression of TNFAIP2 in U87 and U251 cells compared with their corresponding negative control. GAPDH was used as the loading control. Data are shown as mean + s.d. (n = 3); *indicates P-value <0.05, **indicates P-value <0.01.