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Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: MiR-223 promotes the cisplatin resistance of human gastric cancer cells via regulating cell cycle by targeting FBXW7

Figure 2

Down-regulation of miR-223 significantly increases the sensitivity of 7901/DDP cells to DDP. (A) Forty-eight hours after transfection with miR-223 inhibitor or inhibitor NC, qRT-PCR detection of miR-223 expression in cells. U6 was used as an internal control; (B) 7901/DDP cells transfected with miR-223 inhibitor show more DDP sensitivity than cells transfected with inhibitor NC. Indicated miR-223 inhibitor or inhibitor NC-transfected 7901/DDP cells were plated in triplicate and exposed to DDP (0.0 and 4.0ug/ml). The forci formation was indicated; (C) MTT analysis of the IC50 values of DDP in miR-224 inhibitor- or inhibitor NC-transfected 7901/DDP and parental 7901 cells; (D) Flow cytometric analysis of cell cycle in inhibitor NC- or miR-224 inhibitor-transfected 7901/DDP cells combined with DDP (0.0 and 4.0 ug/ml); (E) Flow cytometric analysis of apoptosis in miR-224 inhibitor- or inhibitor NC-transfected 7901/DDP combined with DDP (0.0 and 4.0 ug/ml); Data are expressed as the mean ± S.D. of three individual experiments. *p < 0.05.

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