Figure 3

Up-regulation of miR-223 significantly reduces the sensitivity of 7901 cells to DDP. (A) qRT-PCR detection of miR-223 expression in miR-223 mimic or NC-tranfected 7901 cells. U6 was used as an internal control; (B) 7901/miR-223 cells show less DDP sensitivity than 7901/NC cells. Indicated 7901/miR-223 or 7901/NC cells were plated in triplicate and exposed to DDP (0 and 2.0 ug/ml). The forci formation was indicated; (C) MTT analysis of the IC₅₀ values of DDP in 7901/miR-224 or 7901/NC cells; (D) Flow cytometric analysis of cell cycle in 7901/miR-223 or 7901/NC cells combined with DDP (0 and 2.0 ug/ml); (E) Flow cytometric analysis of apoptosis in 7901/miR-223 or 7901/NC cells combined with DDP (0 and 2.0 ug/ml); Data are expressed as the mean ± S.D. of three individual experiments. (*p < 0.05, **p < 0.01).