Figure 6
siRNA-mediated down-regulation of FBXW7 could mimic effect of miR-223 up-regulation on sensitivity of 7901 cells. (A) Forty-eight hours after transfection with siRNA/FBXW7 or siRNA/NC, qRT-PCR and western blot detection of FBXW7 mRNA and protein expression; (B) MTT analysis of the IC50 values of DDP in siRNA/NC or siRNA/FBXW7-transfected 7901 cells; (C) Flow cytometric analysis of cell cycle in siRNA/NC or siRNA/FBXW7-transfected 7901 cells combined with DDP (0 and 2 ug/ml); (D) Flow cytometric analysis of apoptosis in siRNA/NC or siRNA/FBXW7-transfected 7901 cells combined with DDP (0 and 2 ug/ml). Data are expressed as the mean ± S.D. of three individual experiments. *p < 0.05.