Figure 1

Lactate production and HIF-1α expression in pancreatic cancer cell lines. A). Effect of 2-DG and D-allose alone or in combination with SB202190 following 24 hours of treatment on lactate production measured in cell culture media or in cells. Values are normalized to total cell protein. One representative experiment is shown, n = 3. *p < 0.05 B). Western blot of cells grown in normoxia or hypoxia for 24 and 48 hours treated with SB202190 and analyzed for HIF-1α. Tubulin served as a loading control for protein. HIF-1α band density was quantified using ImageJ software with normalization to α-Tubulin.