Figure 2

HIF-1α transcriptional activity is modulated by SB202190. A). MIA PaCa-2 cells were stably transfected with a HRE-luciferase reporter and treated for 3 24, and 48 hours with 2-DG or D-allose alone and in combination with SB202190. Luciferase activity was normalized to total protein. One representative experiment is shown, n = 3. *Indicates a p < 0.05 relative to vehicle control. X indicates a p < 0.01 relative to SB202190 treated control. B). Effect of glucose analogs alone and in combination with SB202190 after 24 and 48 hours in MIA PaCa-2 cells measured by Western blot analysis of HIF-1α. Tubulin was used as a loading control. Quantification of HIF-1α band density was performed using ImageJ software with normalization to α-Tubulin.