Induction of mBAP31-specific IFN-γ production by T cells following mBAP31 DNA vaccine vaccination in C57BL/6 mice. Three groups (n = 6) of female C57BL/6 mice were immunized subcutaneously at the base of the tail with the specified DNA plasmid (p-mBAP31, p-LAMP/mBAP31) or PBS and were boosted twice every 3 weeks. 2 weeks after the third vaccination, mice were sacrificed and the splenocytes were harvested. The frequency of cells producing IFN-γ in splenocytes was measured by ELISPOT. The number of IFN-γ-producing cells of p-LAMP/mBAP31 group was significantly higher than that of p-mBAP31 and control group.