Figure 5

LDH release assay for cytotoxicity of CTLs from mBAP31 vaccinated mice against murine cancer cell lines. Cytotoxic activities of CTLs isolated from the splenocytes of the p-LAMP/mBAP31 vaccinated C57BL/6 mice (H-2^b) against mBAP31 peptides pulsed mouse colon cancer cell lines CT26 (H-2^d), 2E8 (mBAP31 depleted B16 cells, H-2^b) and B16 (H-2^b) were detected by LDH release assay at effector-to-target ratio of 50:1. Peptides used were indicated in abscissa (A-C). Cytotoxic activities of CTLs from p-LAMP/mBAP31 (△), p-mBAP31 (□) or PBS (○) vaccinated C57BL/6 mice (H-2^b) against B16 cells pulsed with mBAP31_149–157 expressing both H-2^b and mBAP31 (D), 2E8 cells (mBAP31 depleted B16 cells) pulsed with mBAP31_149–157 expressing H-2^b but much lower mBAP31 than B16 cells (E), CT26 cells pulsed with mBAP31_149–157 expressing H-2^d and mBAP31 (F), B16 cells expressing both H-2^b and mBAP31 (G), 2E8 cells expressing H-2^b but much lower mBAP31 than B16 cells (H) and CT26 cells expressing H-2^d and mBAP31 (I).