MET modulation accounts for the antimetastatic effect of miR-144. (A) Western blot showing MET expression in a set of human GC cell lines. (B) A significant inverse correlation is observed between the miR-144 and MET expression levels in the GC tissues (n = 52). (C) The MET and phosphorylated Akt were inhibited by the forced expression of miR-144 or siRNA-MET. (D, E) The effects of miR-144 or siRNA-MET on the migration and proliferation were determined in SNU-5 cells. (F) The MET and phosphorylated Akt were restored by the overexpression of MET in miR-144 mimics-treated SNU-5 cells. (G, H) The effects of miR-144 combined with MET-ORF on the migration and proliferation of SNU-5 cells. (I) The MET and phosphorylated Akt were up-regulated by blocking of miR-144 in AGS cells. (J, K) The effects of anti-miR-144 on the migration and proliferation of AGS cells.