Figure 4

Tumor specific immune response induced by Ub-enriched proteins and DRibbles. C57/BL6 mice (3 mice/group) were primed by 30 μg i.n and twice 100 μg s.c vaccination with two days of interval by Ub-enriched proteins and Dribbles respectively isolate from EL4 cells A) and B16-F10 cells B). Ten days after the last vaccination, Both lymph nodes and spleen of the mice were collected, processed into single cell suspensions and re-stimulated by EL4 and B16 tumor cells that already inactivated by 10 nM of colchicines or without stimulation (CM). α -CD3-Ab used as positive control. The secreted IFN-γ level was determined after 72 h by ELISA. Data are representative of three independent experiments results.