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Figure 6 | Journal of Experimental & Clinical Cancer Research

Figure 6

From: Short hairpin RNA- mediated gene knockdown of FOXM1 inhibits the proliferation and metastasis of human colon cancer cells through reversal of epithelial-to-mesenchymal transformation

Figure 6

Effect of altered FOXM1 expression on colorectal cancer cell migration and invasion in vitro. (A): Wound healing assays were carried out at 24h after transfection in 24-well plates, when cell confluence rate reached above 90% and a linear wound across the monolayer was done. The wound gap was photographed every 24h, the gap width was measured (μm) using Open Lab software. (B): the wound rate was calculated and displayed graphically as described in the Materials and Methods. (C-D): SW620 cells of three groups were digested and resuspended in serum-free culture medium and allowed to migrate toward the lower chamber with coated or uncoated matrigel for 24h. Invading cells were stained with 0.1% crystal violet and counted manually. C-Left: transwell migration assay, Right: transwell invasion assay. (D): The number of invading SW620 cells by cell migration (upper) and invasion (lower) assay was counted manually. Each experiment was repeated thrice independently. Scale bar = 200μm in those figures.

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