Figure 5

Induction of FOXO3a and RUNX3 mediated baicalein-inhibited cell growth. A-B, H1650 cells were transfected with control or FOXO3a, or RUNX3 siRNAs with lipofectamine 2000 reagent for 24 h, followed by exposure the cells to baicalein (BA, 75 μM) for an additional 24 h. Afterwards, the cells proliferation was detected using MTT assays. The expression of FOXO3a and RUNX3 protein was determined by Western blot. The bar graphs represent the mean ± SD of RUNX3/GAPDH and FOXO3a/GAPDH of three independent experiments. The insert blots represent the protein expression of FOXO3a and RUNX3. C-D, H1650 cells were transfected with control or FOXO3a or RUNX3 siRNAs (50 nM each) for 24 h before exposing the cell to baicalein (BA) for an additional 24 h. Afterwards, the expression of FOXO3a and RUNX3 protein was determined by Western blot. *indicates significant difference from untreated control cells **indicates significant difference from baicalein treated alone (P < 0.05).