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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Increased HMGB1 and cleaved caspase-3 stimulate the proliferation of tumor cells and are correlated with the poor prognosis in colorectal cancer

Fig. 1

HMGB1 stimulated cell proliferation after irradiation. (a) The irradiated HT29 cells (feeder cells) significantly stimulated HT29 Fluc labeled cells growth compared to non-irradiated HT29 feeder cells or reporter alone cells (No feeder). **p <0.01. (b) Cell death detection by flow cytometry. (c) Western blot of correlated expressions of HMGB1 and PCNA protein in colon cancer cells. Relative intensity changes of HMGB1 and PCNA were analyzed through gray value using software ImageJ. Positive correlation between HMGB1 expression and proliferative marker was observed in colon cell lines. (d) Western blot of HMGB1, PCNA, and caspases-3 levels in HT29 and HCT8 cells. Cells were irradiated at 10 Gy. The supernatant HMGB1 was elevated significantly in both cell lines, while there was no obvious change of intracellular HMGB1. PCNA and increased caspases-3 cleavage were also observed after irradiation

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