Fig. 6

miR-29b directly targets 3’-UTR of the MMP2 gene. a, Predicted binding sites in the 3’-UTR of MMP2 mRNA and seed sequence of miR-29b by TargetScan. b, Quantitative RT-PCR analysis of miR-29b expression levels in A549 and H460 cell lines after infection LV-miR-29b or miR-29b inhibitor, respectively. c, Quantitative RT-PCR analysis of MMP2 mRNA expression levels in A549 and H460 cell lines after infection LV-miR-29b or miR-29b inhibitor, respectively. d, Western blots analysis of MMP2 expression levels in A549 and H460 cell lines following LV-miR-29b or miR-29b inhibitor infection, respectively. e, Dual luciferase activity indicating relative luciferase activity following co-transfection with psiCHECK-2-Wt-MMP2-3’UTR or psiCHECK-2-Mut-MMP2-3’UTR and miR-29b mimic. Results are presented as means ± SEM (*P < 0.05, n = 3)