Fig. 7From: T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell linesNNC-55-0396 exposure increased intracellular baseline Ca2+ levels via inducing ER calcium release. Jurkat T cells stained with Fluo-4 were preincubated with 1–10 μM NNC-55-0396 in the absence of extracellular Ca2+. For each sample, after the 10 min pretreatment with different concentrations of NNC-55-0396 baseline Ca2+ measurements were taken, cells were then stimulated at the 2 min mark with 1 μM thapsigargin (TG) to induce the endoplasmic reticulum (ER) Ca2+ release, and the analysis was immediately resumed. The results depicted are representative of three independent experimentsBack to article page