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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Gastric cancer-derived mesenchymal stem cells prompt gastric cancer progression through secretion of interleukin-8

Fig. 6

Pro-angiogenesis and tumor-promoting effect of GC-MSC-secreted IL-8 on MKN-28 cells. (A) RT-PCR analysis of VEGF, MIP-2, TGF-β1, IL-6, and IL-8 expression in GC-MSCs, GCN-MSCs, or BM-MSCs after exposed to 10 % MKN-28-CM for 3 days. (B) RT-PCR analysis of VEGF, MIP-2, TGF-β1, IL-6, and IL-8 expression in MKN-28 cells after treated with 10 % CM from GC-MSCs, GCN-MSCs, or BM-MSCs for 3 days. (C) Representative photographs of HUVECs seeded on Matrigel for 8 hrs in culture medium and in the presence of 10 % CM from GC-MSCs, MKN-28 cells, or co-cultured MKN-28 cells and GC-MSCs (×100). (D) Viability of MKN-28 cells cultured in 10 % GC-MSC-CM treated with or without anti-IL-8 antibody by MTT assay. (E) Transwell migration assay of MKN-28 cells exposed to 10 % GC-MSC-CM with or without anti-IL-8 antibody treatment (×200). (F) Expression of VEGF, MIP-1, IL-6, and IL-8 in MKN-28 cells exposed to 10 % GC-MSC-CM with or without IL-8 blockade. (G) Western blot for protein levels of Akt, p-Akt, p44/42 MAPK (Erk1/2), and p-p44/42 MAPK (Erk1/2) in MKN-28 cells stimulated by 10 % GC-MSC-CM with or without IL-8 blockade

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