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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Gastric cancer-derived mesenchymal stem cells prompt gastric cancer progression through secretion of interleukin-8

Fig. 7

Tumor-promoting effect of GC-MSCs on BGC-823 cells is attenuated by the application of anti-IL-8 antibody. (A) Cytokine profile analysis of GC-MSCs, GCN-MSCs, or BM-MSCs by Luminex immunoassay. (B) Viability of BGC-823 cells cultured in 10 % GC-MSC-CM treated with or without anti-IL-8 antibody by MTT assay. (C) Transwell migration assay of BGC-823 cells exposed to 10 % GC-MSC-CM with or without anti-IL-8 antibody treatment (×200). (D) Expression of VEGF, MIP-1, IL-6, and IL-8 in BGC-823 cells exposed to 10 % GC-MSC-CM with or without IL-8 blockade. (E) Western blot for protein levels of Akt, p-Akt, p44/42 MAPK (Erk1/2), and p-p44/42 MAPK (Erk1/2) in BGC-823 cells stimulated by 10 % GC-MSC-CM with or without IL-8 blockade. (F) Representative photographs of capillary tube formation by HUVECs exposed to 10 % GC-MSC-CM with or without IL-8 blockade. (×100). *P < 0.05, **P < 0.01: compared with the control group; # P < 0.05, ## P < 0.01: compared with the GC-MSC-CM treated group

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