Table 1 Primer sequences for the amplification of target genes
Genes | Accession number | Primer sequence (5′–3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
VEGF | NM_001025366 | For: 5′-TTGCCTTGCTGCTCTACCTC-3′ | 198 | 60 |
|  |  | Rev: 5′-CACAGGATGGCTTGAAGATG-3′ |  |  |
MIP-2 | NM_002089 | For: 5′-AACCGAAGTCATAGCCACAC-3′ | 150 | 59 |
|  |  | Rev: 5′-CAGGAACAGCCACCAATAAG-3′ |  |  |
TGF-β1 | NM_000660 | For: 5′-CACGTGGAGCTGTACCAGAA-3′ | 114 | 61 |
|  |  | Rev: 5′-CACAACTCCGGTGACATCAA-3′ |  |  |
IL-6 | NM_000600 | For: 5′-GAGGAGACTTGCCTGGTGAA-3′ | 267 | 60 |
|  |  | Rev: 5′-GCGCAGAATGAGATGAGTTG-3′ |  |  |
IL-8 | NM_000584 | For: 5′-ACCGGAAGGAACCATCTCAC-3′ | 822 | 61 |
|  |  | Rev: 5′-GTGGATCCTGGCTAGCAGAC-3′ |  |  |
β-actin | NM_001101 | For: 5′-TGGACTTCGAGCAAGAGATG-3′ | 207 | 60 |
|  |  | Rev: 5′-GGATGTCCACGTCACACTTC-3′ |  |  |