Fig. 1From: Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemiaAssay design of ASB-PCR. Primers and probe are located in exon 23 of DNMT3A. The allele-specific primer contains the mutational spot at its 3′-end whereas the wt spot is incorporated in the middle of the blocker (red box). Fluorescence detection was performed with a TaqMan probe which was designed near to the forward primer (13 bp distance)Back to article page