Fig. 2
From: Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemia
Performance of ASB-PCR. ai Qualitative analysis of the specificity of ASB-PCR. Enhancement of blocker concentration to 80 μM showed no significant change in the amplification properties. aii Quantitative analysis of Ct-changes induced by addition of 40 μM blocker. b Quantitative analysis displayed a Ct difference of 11–14 cycles between R882H mutated DNA and wt DNA. NTC, non-template control; wt, wild type