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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemia

Fig. 5

Absolute quantification of DNMT3A R882H mutations. Values of ASB-PCR are listed in the corresponding table. a Analysis of copy number dilutions of the R882H homozygote plasmid. Patient samples with approximately 50 % of R882H mutated allele are indicated by dotted lines; the 10° plasmid dilutions are pink line. b The standard curve (copy numbers over cycle). Patient samples with 50 % R882H are shown as a blue diamond and a green triangle. Wt samples are shown as red quadrats. R2 = correlation coefficient. B = Intercept with the ordinate. M = Slope of the standard curve. c Absolute quantification was performed by percentage of mutated allele in a sample. Plasmid concentrations were adjusted to 50 % and 25 % of mutated R882H allele according to the measurement of heterozygote patient samples (dotted lines). d Standard curve (%R882H over cycle). The dotted red line shows the limit of 100 % R882H mutated allele in a sample. Patient samples are shown as a blue diamond and a green triangle. wt samples are shown as red quadrats. R2 = correlation coefficienct. B = Intercept with the ordinate. M = Slope of the standard curve

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