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Table 1 Oligonucleotides used in this study

From: Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemia

Name Sequence Application Tm [°C]
DNTM3A-Ex23F 5′-GTGTGGTTAGACGGCTTCC Sequencing 59.5
DNMT3A-Ex23R 5′-CTCTCCCACCTTTCCTCTG Sequencing 59.5
ASB-F 5′- CAGCGGAGCGAAGAGGTG ASB-PCR 60.8
Allele-specific 5′- CGTCTCCAACATGAGCCA ASB-PCR 56.3
ASB-Blocker 5′- CATGAGCCGCTTGGCGAG-PH ASB-PCR 60.8
ASB-Probe 5′- FAM-CTCCATGACCGGCCCAGCAGTC-BBQ ASB-PCR 69.5
  1. The mutational spot in the allele-specific primer and blocker sequence is bold and punctuated. ASB, indicates Allele-Specific PCR with a Blocking reagent; DNMT3A, indicates DNA methyltransferase 3 gene