Table 1 Oligonucleotides used in this study
From: Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemia
Name | Sequence | Application | Tm [°C] |
|---|---|---|---|
DNTM3A-Ex23F | 5′-GTGTGGTTAGACGGCTTCC | Sequencing | 59.5 |
DNMT3A-Ex23R | 5′-CTCTCCCACCTTTCCTCTG | Sequencing | 59.5 |
ASB-F | 5′- CAGCGGAGCGAAGAGGTG | ASB-PCR | 60.8 |
Allele-specific | 5′- CGTCTCCAACATGAGCCA | ASB-PCR | 56.3 |
ASB-Blocker | 5′- CATGAGCCGCTTGGCGAG-PH | ASB-PCR | 60.8 |
ASB-Probe | 5′- FAM-CTCCATGACCGGCCCAGCAGTC-BBQ | ASB-PCR | 69.5 |