Fig. 1

Salinomycin inhibits proliferation and survival of cultured glioma cells in vitro. U87MG glioma cells were treated with indicated concentration of salinomycin (Sali) for 72 h, or treated with 5 μM of salinomycin (Sali) for indicated time, cell viability and cell death were analyzed by MTT assay (a and b) and trypan blue staining (c) respectively. U87MG cells were cultured in salinomycin (Sali, 5 μM) containing medium for 10 days, and the left survival colonies were counted, representative images were also shown (d). U251MG and EFC-2 glioma cells were treated with salinomycin (Sali, 5 μM) for 72 h; cell viability was tested by MTT assay (e). Primary mouse astrocytes were treated with indicated concentration of salinomycin (Sali) for 72 h, cell morphology and viability were shown in (f) and (g). Experiments were repeated four times in this figure, and similar results were obtained. Error bars indicate standard deviation (SD). *p < 0.05 vs. untreated control (“c”) group. Magnification 1:400 for (f)