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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: ROS-p53-cyclophilin-D signaling mediates salinomycin-induced glioma cell necrosis

Fig. 3

Cyp-D is required for salinomycin-induced necrosis but not apoptosis in cultured glioma cells. U87MG cells were pre-treated with the Cyp-D inhibitor CsA (0.1 μM) or SfA (10 μM) for 1 h, followed by salinomycin (Sali, 5 μM) stimulation, cells were further cultured, MTT assay (a, 72 h) and trypan blue staining (d, 72 h) were performed. U87MG cells transfected with scramble or Cyp-D siRNA (100 nM each, 48 h) were treated with salinomycin (Sali, 5 μM) for 72 h, cell viability and death were tested (c and d), expression of Cyp-D and tubulin was shown (b). Stable U87MG cells with empty vector (p-Super) or Cyp-D-GFP (1 μg/ml each) were treated with salinomycin (Sali, 1 or 5 μM) for 72 h, Cyp-D/tubulin expression (e) and cell death (f) were tested. Non-transfected control U87MG cells, Cyp-D siRNA-transfected U87MG cells or Cyp-D-over-expressing U87MG cells were treated with salinomycin (Sali, 5 μM) for 72 h; cell apoptosis (g) and necrosis (h) were tested by FACS. Experiments were repeated three times in this figure, and similar results were obtained. Error bars indicate SD. *p < 0.05 vs. dmso group (a and d). *p < 0.05 (c and f)

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