Fig. 2

Immunohistochemical staining of Dppa4 expression in tissue microarrays. a Negative-Dppa4 expression in normal colonic epithelium and (b) Diffuse, intense Dppa4 staining in moderately and poorly-differentiated colon tumors; (c) colon cancer lymph node metastasis. Original magnification × 200 (×50 for inserts). (d) Dppa4 expression in 185 cases of paired normal colon tissue, primary colon cancer and 63 cases of lymph node metastasis (LNM). Dppa4 expression was significantly higher in LNM than in primary colon cancer and normal colon tissue. (P < 0.001)