Fig. 2

Knockdown of YY1 in melanoma cells inhibits cell proliferation, migration and invasion. (a) YY1 level were detected in WM1791C and WM209 cells after treatment with siRNAs specific to YY1 (25 nM) or siRNA control (25 nM) by RT-qPCR; (b) Cell proliferation assay of WM1791C and WM209 cells after treatment with si-YY1 or si-control by using CCK-8; (c) Cell cycle analysis by propidium iodide (PI) staining of WM1791C and WM209 cells after treatment with si-YY1 or si-control; (d) Wound healing assays of WM1791C (the left panel) and WM209 (the right panel) cells after treatment with si-YY1 or si-control, The relative ratio of wound closure per field was shown in the below; (e) Transwell analysis of WM1791C cells after treatment with si-YY1 or si-control, The relative ratio of invasive cells per field was shown below. For the quantitative results, the data are presented as the mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments. *, p < 0.05; **, p < 0.01