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Table 2 Summary of EGFR exons 18–21 mutations in 494 NSCLC-FFPE tissues and KRAS exon 2 mutations in 1099 CRC-FFPE tissues as detected by our designed pyrosequencing method

From: The mutation rates of EGFR in non-small cell lung cancer and KRAS in colorectal cancer of Chinese patients as detected by pyrosequencing using a novel dispensation order

Gene Exon Type of mutation Nucleotide change Amino acid change No. with mutation (%)
EGFR 19 Missense c.2239TT>CC p.L747P 1 (0.20)
  19 Deletion c.2235_2249del15 p.E746_A750 53 (10.73)
    c.2236_2250del15 p.E746_A750 18 (3.64)
    c.2237_2251del15 p.E746_T751 2 (0.40)
    c.2237_2253del17 p.E746_T751 1 (0.20)
    c.2237_2255del19 p.E746_S752 1 (0.20)
    c.2237_2257del21 p.E746_P753 1 (0.20)
    c.2239_2248del10 p.L747_E749 2 (0.40)
    c.2239_2253del15 p.L747_T751 1 (0.20)
    c.2239_2256del18 p.L747_S752 2 (0.40)
    c.2240_2254del15 p.L747_T751 2 (0.40)
    c.2240_2257del18 p.L747_P753 5 (1.01)
    c.2253_2276del24 p.S752_I759 3 (0.61)
    c.2254_2277del24 p.S752_I759 1 (0.20)
  21 Missense c.2573T>G p.L858R 81 (16.40)
    c.2582T>A p.L861Q 2 (0.40)
Total      176 (35.63)
KRAS 2 Missense c.34G>T p.G12C 24 (2.18)
    c.34G>A p.G12S 26 (2.37)
    c.34G>C p.G12R 1 (0.09)
    c.35G>A p.G12D 168 (15.29)
    c.35G>C p.G12A 23 (2.09)
    c.35G>T p.G12V 85 (7.73)
    c.34G>A + c.35G>A p.G12S + p.G12D 6 (0.55)
    c.37G>T p.G13C 4 (0.36)
    c.38G>A p.G13D 100 (9.10)
Total      437 (39.76)
  1. EGFR epidermal growth factor receptor, KRAS V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, NSCLC non-small cell lung cancer, CRC colorectal cancer, FFPE formalin-fixed paraffin-embedded