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Table 2 Summary of EGFR exons 18–21 mutations in 494 NSCLC-FFPE tissues and KRAS exon 2 mutations in 1099 CRC-FFPE tissues as detected by our designed pyrosequencing method

From: The mutation rates of EGFR in non-small cell lung cancer and KRAS in colorectal cancer of Chinese patients as detected by pyrosequencing using a novel dispensation order

Gene

Exon

Type of mutation

Nucleotide change

Amino acid change

No. with mutation (%)

EGFR

19

Missense

c.2239TT>CC

p.L747P

1 (0.20)

 

19

Deletion

c.2235_2249del15

p.E746_A750

53 (10.73)

   

c.2236_2250del15

p.E746_A750

18 (3.64)

   

c.2237_2251del15

p.E746_T751

2 (0.40)

   

c.2237_2253del17

p.E746_T751

1 (0.20)

   

c.2237_2255del19

p.E746_S752

1 (0.20)

   

c.2237_2257del21

p.E746_P753

1 (0.20)

   

c.2239_2248del10

p.L747_E749

2 (0.40)

   

c.2239_2253del15

p.L747_T751

1 (0.20)

   

c.2239_2256del18

p.L747_S752

2 (0.40)

   

c.2240_2254del15

p.L747_T751

2 (0.40)

   

c.2240_2257del18

p.L747_P753

5 (1.01)

   

c.2253_2276del24

p.S752_I759

3 (0.61)

   

c.2254_2277del24

p.S752_I759

1 (0.20)

 

21

Missense

c.2573T>G

p.L858R

81 (16.40)

   

c.2582T>A

p.L861Q

2 (0.40)

Total

    

176 (35.63)

KRAS

2

Missense

c.34G>T

p.G12C

24 (2.18)

   

c.34G>A

p.G12S

26 (2.37)

   

c.34G>C

p.G12R

1 (0.09)

   

c.35G>A

p.G12D

168 (15.29)

   

c.35G>C

p.G12A

23 (2.09)

   

c.35G>T

p.G12V

85 (7.73)

   

c.34G>A + c.35G>A

p.G12S + p.G12D

6 (0.55)

   

c.37G>T

p.G13C

4 (0.36)

   

c.38G>A

p.G13D

100 (9.10)

Total

    

437 (39.76)

  1. EGFR epidermal growth factor receptor, KRAS V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, NSCLC non-small cell lung cancer, CRC colorectal cancer, FFPE formalin-fixed paraffin-embedded