Fig. 3

HF effects on the angiogenic factors in endothelial cell HUVEC. a Cells were treated with 50, 100 or 200 ng/mL of HF for 24 hours and mRNA expression of angiogenic factors was assessed by real time PCR. Graphic shows differential expression compared to non-treated cells (Fold change = 1). b Cells were treated for 72 hours to quantify VEGF production in the supernatant. CTRL: non-treated cells. ***P < 0.0001; (one-way ANOVA). Data are representative of two independent experiments (error bars, s.e.m.)