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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: microRNA-93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells

Fig. 2

miR-93 inhibits PTEN mRNA expression in Saos cells and silencing of PTEN with anti-miR-93 in OS cells. a Predicted binding sites of miR-93 at the 3′UTR site of PTEN, as aligned by Target Scan and BLAST. b Scheme and sequence of the intact miR- 93 (Wt) and its mutant (Mut). (c) After actinomycinD treatment, the miRNA expression level of miR-93 in the negative control-miR, miR-93 and miR-93 mutant was measured by qRT-PCR. d After actinomycinD treatment, the mRNA expression level of PTEN after transfection of negative control-miR, miR-93 and miR-93 mutant was measured by qRT-PCR. e Up-regulation of PTEN using anti- miR-93 and PTEN-expression vector in ES cells. f Densitometry quantification of PTEN protein after transfection of anti-miR-93. g Transfection of PTEN expression vector in ES cells increases the expression of PTEN protein. h The quantification of PTEN protein after transfection of PTEN expression vector. ANOVA was carried out to statistically analyze the results. (**) P < 0.01

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