Fig. 5
From: microRNA-93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells
Effects of anti-miR-93 and PTEN expression vectors on the cell cycle distribution in Saos. Cells were treated and analyzed by flow cytometry after staining with PI (a-d). Histogram shows quantitative percentage of diploid cells (DNA content) in each cell cycle phase (e). Effects of anti-93-miR and PTEN vector on induction of apoptosis in Saos. Western blot showed the expression of PARP and its cleaved form (f). Transfection of anti-93-miR and PTEN plasmid with low dose Adriamycin showed the expression of PARP and its cleaved form Saos (g). The cells were labelled with FITC annexin V and PI in oligo transfection (h) and oligo transfection with low dose Adriamycin (i). Each quadrants represent viable cells (Lower Left quadrant), early apoptotic cells (Lower Right), late or secondary necrotic cells (Upper Right), and primary necrotic cells (Upper Left), respectively