Fig. 5

Regulation of Bcl-2 by MEG3. a Immunoblot analysis of Bcl-2 in HGC-27 cells transfected with miR-181a inhibitor or inhibitor control; An unrelated protein GAPDH was used as the control. b The expression of MEG3 and Bcl-2 mRNA in HGC-27 cells transfected with empty vector, wild type MEG3 or mutant MEG3 as indicated; c Immunoblot analysis of Bcl-2 protein in HGC-27 cells transfected with empty vector, wild type MEG3 or mutant MEG3 as described in B; d A Schematic model of MEG3/miR-181/Bcl-2 cascade in gastric carcinogenesis. For all quantitative results, the data are presented as the mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments.*p < 0.05; **p < 0.01