Fig. 5
From: Drug-releasing mesenchymal cells strongly suppress B16 lung metastasis in a syngeneic murine model
Sca-1+ cells in lung parenchyma of SR4987PTX injected mice localize in vessels nearby metastatic nodule. Anti-Sca-1 antibodies were used to investigate the capacity of SR4987 and SR4987PTX to home lung B16 nodules. a quantification of Sca-1 + cells in the lungs. Bars are the mean ± SD of three mice and are the number of Sca-1+cells/mm2 counted at 400X (**p < 0.01 vs CTRL or PTX treated mice). b,c,f,h Immunostaining for Sca-1 of metastatic lung parenchyma and (d,e,g,i) metastasis (m) area. Very few Sca-1+ cells (white arrows) are present in the lung of control and PTX treated mice in both parenchyma (b,c) and metastasis area (d,e). Mice treated either with SR4987 (f,g) and SR4987PTX (h,i) show many Sca-1+ cells in the lung parenchyma near the vessels (V) and at the periphery of metastatic nodules (200x magnifications). (J) Fontana-Masson staining showing B16 metastasis nearby (top left panel) or surrounding a lung microvessel (bottom right panel). In (L,M) Sca-1+ cells (black arrows) are attached to endothelium of tumor infiltrating microvessels