Fig. 3From: Targeting sphingosine kinase 2 (SphK2) by ABC294640 inhibits colorectal cancer cell growth in vitro and in vivo The cytotoxic effect of ABC294640 in primary human CRC cells. Ex vivo cultured primary cancer cells derived from three different colon cancer patients (−1, male, 47 yeas old; −2, female, 62 years old; −3, male, 55 years old) were treated with or without ABC294640 (1 μM) for 72 hours, cell growth and cell death were tested by MTT assay (b) and LDH release assay (c), respectively. Expression of SphK2 and tubulin in above cells was also shown (a). Mean values ± SD of three independent experiments were reported. Statistical analysis was performed comparing treatment groups with vehicle control group (“C”). *P < 0.05Back to article page