Fig. 4

Signaling changes in ABC294640-treated CRC cells. HT-29 cells or primary CRC cells were treated with or without ABC294640 (1/3 μM) for 12 hours, listed proteins were tested by Western blots (a-d). HT-29 cells were pre-treated with SP600125 (5 μM) or JNK inhibitor II (JNKi-II, 5 μM) for 1 hour, followed by ABC294640 (1 μM) stimulation, cell growth (e) and cell apoptosis (f) were analyzed. Mean values ± SD of three independent experiments were reported. Statistical analysis was performed comparing treatment groups with vehicle control group (“C”). *P < 0.05. ^#P < 0.05 vs. ABC294640 only group (e and f)